Coding
phaC2

Part:BBa_K2434003:Design

Designed by: Diana Priscilla Bonilla Ruelas   Group: iGEM17_TecMonterrey_GDA   (2017-09-23)


phaC2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1588
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 877
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1287


Design Notes

This sequence has been optimized for E.coli and a codon was changed from CAG to CAA to avoid restriction site PstI.


Source

https://www.ncbi.nlm.nih.gov/gene/?term=881185 This sequence comes from the Pseudomonas aeruginosa PAO1.

References